Membrane-Protein Expression Scientist

PARIS, FRANCE          FULL-TIME

At Eligo, we are passionate about developing new drugs to address unmet medical needs!

Eligo is building a platform technology to produce eligobiotics that can target a wide range of bacterial species. With such a capability, Eligo is poised to lead the new generation of precision microbiome engineering companies.

We are looking for excellent protein expression scientists excited to tackle the ambitious challenges of diving into exogenous protein expression on the membrane of specific bacterial strains. This involves cloning and testing engineered DNA constructs, expression of folded outer membrane proteins in bacteria, handling of bacterial cultures and bacteriophages and meticulously recording and interpreting results.

If you are thoughtful, meticulous, well-organized, energetic, collaborative, and want to learn about and provide support for a critical set of processes within Eligo, please apply!

Responsibilities

  • Protein Engineering : design and construction of different full-length outer membrane proteins and fusion variants.
  • Application of Bioengineering methods to design, build, test and debug novel and complex DNA circuits using advanced molecular cloning techniques (Golden Gate, Gibson assembly, etc.)
  • Generation of bacteriophage stocks and biocarrier-encapsulated DNA payloads as well as their titration and other forms of quantification.
  • Delivery of engineered DNA circuits into the target bacteria : transformation, biocarrier-mediated transduction.
  • Testing viability of engineered DNA circuits in bacteria : fluorescence measurements, CFU counting, OD600 growth curves, etc.
  • Carefully quantifying expression levels of DNA circuit payloads in target bacteria by different methods : immunohistochemistry, flow cytometry, ELISA, etc.
  • Record, perform calculations and interpret results from experiments and outcomes as well as troubleshooting to optimize DNA circuit and fusion protein design.
  • Track progress of all results using an electronic lab notebook.
  • Bi-weekly reporting of results.

Requirements

  • PhD in Protein Science or related discipline (Protein Biochemistry, structural Biology, etc.)
  • Deep knowledge of membrane protein expression, ideally in bacteria: extended knowledge of export pathway mechanisms, quantification of expression levels, generation of chimeric variants directed to the outer membrane, methods to improve solubility and proper folding, etc.
  • Protein engineering experience: generation of protein fusions, truncated variants.
  • Experience with protein biochemistry: ligand/antibody binding assays, colorimetry, etc.
  • Advanced molecular cloning: Gibson, Golden Gate, iPCR.
  • Quantification of protein expression and solubility: fluorescence, ELISA, Western Blot, SDS-PAGE, Bradford/BCA/other methods for protein quantification, subcellular fractionation, etc.
  • Immunohistochemistry skills in both fixed and living cells.
  • Experience with flow cytometry/FACS is preferred but not essential.
  • Knowledge of bacteriophage biology is an advantage.
  • Understanding the basic principles of genetic circuit engineering is a plus but not strictly required: promoter strengths, RBS libraries, codon balance.
  • Basic computer skills required: word processing and spreadsheets.
  • Must be capable of organizing work, multi-tasking, and performing multiple functions that may change over time.
  • Excellent attention to detail and ability to resolve problems.
  • Must be receptive to instruction and demonstrate aptitude for learning new technologies.
  • Good oral and written communication skills in English required.
  • Must work well independently and also as part of a team.