Internship in Molecular- and Microbiology of Anaerobes
Eligo is seeking an excellent molecular- and microbiology intern to help expand our collection of new bacteria and isolate bacteriophages, as well as assist with the development of high-throughput pipelines and processes to streamline these tasks.
The intern will join the Discovery & Automation team and help us advance a variety of exciting projects. They will gain significant experience in laboratory bench-top techniques and their deliverables will have direct and meaningful impacts on Eligo’s drug projects.
About Eligo: At Eligo, we are passionate about developing new drugs to address unmet medical needs! Eligo is a biotech company building a platform technology to enable the precise engineering of the human microbiome, targeting a wide range of bacterial species. To unlock our ability to target key members of the microbiome, we apply our discovery and engineering platform to an increasing number of bacterial species.
— Applicant profile and internship conditions —
The ideal intern should be well-prepared to quickly learn or improve on the skills required to contribute to the following projects.
Bacterial strain isolation
The intern will assist in isolating fastidious anaerobic bacterial species from complex samples.
→ These efforts involve material preparation (such as growth media), sample processing in an anaerobic environment, careful isolate tracking, molecular biology such as PCR and preparation of DNA for sequencing, analysis of sequencing results, and bacterial strain growth and cryostock production.
The intern will assist in isolating and characterizing bacteriophages against fastidious anaerobic bacteria.
→ These efforts involve material preparation (such as growth media), sample processing (such as filtration and concentration of environmental samples), careful tracking of the samples, molecular biology such as PCR and ddPCR and analysis of results, bacterial strain growth (in liquid culture and on agar plates), transformations, phage plaque isolation and propagation and phage stocks, phage host-range screening, and DNA extraction and preparation for sequencing.
Bonus project: Automation
Depending on the needs of the projects and their skillset, the intern could assist in the development or use of an automation platform (liquid- and plate-handling robot) for increasing the throughput and standardizing the workflows for bacterial strain isolation and phage hunting.
→ These efforts require notions of computer programming.